Ecology & Ecosystems – A-Level Biology Revision Notes
Complete revision notes on ecosystems, energy transfer (GPP & NPP), succession, the nitrogen cycle, eutrophication, sampling methods and conservation. Written by a Chartered Biologist and former WJEC/Eduqas & Edexcel examiner who has marked thousands of ecology questions and knows exactly what earns each mark.
Last updated: February 2026
Why Ecology Catches Students Out – An Examiner’s Perspective
Ecology looks like one of the more accessible topics in A-Level Biology. The language feels familiar – food chains, habitats, populations – and students often assume they can rely on general knowledge. This is precisely why ecology questions produce some of the worst answers I see as an examiner.
The problem is not complexity. It is precision. Ecology questions are built around causal chains where every step must cause the next. When a student writes about eutrophication, I am looking for a sequence of six or seven linked events, each following logically from the previous one. Skipping a step, reversing the order, or using vague language costs marks immediately. The same applies to succession, energy transfer calculations, and the nitrogen cycle.
Having marked ecology questions for both WJEC/Eduqas and Edexcel, I can tell you that the Eduqas 2024 examiner report flagged exactly this: students could not express ecological definitions precisely, struggled to relate NPP to photosynthesis, and many still found basic percentage calculations challenging. The OCR A 2023 report highlighted similar problems with applying knowledge to unfamiliar ecological contexts and choosing appropriate statistical tests.
On this page, I am going to walk you through every ecology topic your exam board requires – and more importantly, I am going to show you how each area is marked. This is not textbook summary. This is what I look for when I am sitting with a mark scheme and a pile of scripts.
Key Ecological Terminology – Definitions That Earn Marks
Every ecology question starts with terminology. Examiners test definitions directly and penalise imprecise language in extended responses. The Eduqas 2024 examiner report specifically flagged that students could not express ecological definitions from Component 1 accurately in Component 2 – synoptic recall of these terms is essential.
Ecosystem
All the organisms (the community) living in a particular area together with the abiotic (non-living) factors of their environment. An ecosystem includes both biotic and abiotic components – this is the critical distinction from “community.”
Community
All the populations of different species living and interacting in the same area at the same time. A community is biotic only – it does not include abiotic factors.
Population
All the organisms of one species living in a particular area at a particular time. A population is always a single species – “the population of organisms in a pond” is incorrect.
Habitat
The place where an organism lives. A habitat provides the conditions and resources that a species needs to survive.
Ecological Niche
The role of a species within its ecosystem, including its interactions with other organisms and its use of abiotic resources. Two species cannot occupy exactly the same niche in the same habitat – this is the competitive exclusion principle.
Abiotic Factors
Non-living physical and chemical conditions that affect organisms: temperature, light intensity, water availability, pH, mineral ion concentration, wind speed, oxygen concentration. Edexcel B also names edaphic factors (soil conditions) as a specific abiotic category.
Biotic Factors
Living factors that affect organisms: predation, competition (interspecific and intraspecific), disease, food availability, parasitism, mutualism.
Energy Transfer Through Ecosystems – GPP, NPP and Efficiency
Energy transfer is one of the most calculation-heavy areas of ecology and one where examiners apply very strict marking conventions. You need to understand the equations, the correct units, and – critically – the correct language. Saying energy is “produced” or “lost” will cost you marks.
The Core Equations
Light energy enters an ecosystem through photosynthesis. The total amount of energy fixed by producers (converted from light energy to chemical energy in organic molecules) is called gross primary productivity (GPP). Producers use some of this energy for their own metabolic processes – primarily respiration. The energy that remains, stored in plant biomass, is called net primary productivity (NPP).
For consumers, the equivalent equation describes how much energy is incorporated into new biomass at each trophic level:
Why Only ~10% of Energy Transfers Between Trophic Levels
The efficiency of energy transfer between trophic levels is typically around 10%. This is because energy is transferred to the surroundings as heat during respiration at every trophic level, some material is not consumed (roots, bones), and some is lost in faeces and urine (material that was ingested but not assimilated).
Farming Practices and Energy Efficiency (AQA Specific)
AQA specifically asks how farming practices increase the efficiency of energy transfer. The key principle is reducing respiratory losses and preventing energy transfer to competing organisms:
- Restricting movement of livestock reduces energy transferred as heat through respiration (less muscle contraction)
- Keeping animals warm reduces energy transferred as heat to maintain body temperature (less thermoregulation)
- Removing competing organisms through pesticides and herbicides ensures more energy from producers reaches the target consumer
- Simplifying food webs – monocultures reduce the number of trophic levels, meaning less energy is transferred to the surroundings at each step
Ecological Succession – How Examiners Mark Each Step
Succession questions consistently appear as high-mark extended response questions (4–6 marks) on every exam board. Having marked hundreds of these, I can tell you that the difference between a Level 1 answer and a Level 3 answer is always the same: mechanism versus description. Students who describe what happens score poorly. Students who explain the mechanism – how each stage causes the next – score full marks.
Primary Succession – The Full Sequence Examiners Expect
Primary succession begins on bare, lifeless substrate with no soil – volcanic rock, new islands, exposed rock faces, sand dunes. The mark scheme expects you to follow this logical chain:
Pioneer Species Colonise
Lichens and mosses are the first organisms to colonise bare rock. They can survive extreme conditions with minimal nutrients. Name them specifically – “small plants” earns nothing.
Pioneers Change Abiotic Conditions
Lichens weather rock surfaces through acid secretion, creating crevices. When pioneers die, their remains decompose to form a thin layer of humus (organic matter). This is the critical mechanism mark – you must explain HOW pioneers alter the environment.
Soil Formation Enables New Species
As humus accumulates, soil deepens. It retains more water and contains more mineral ions. These changed conditions allow grasses and small herbaceous plants to establish – species that could not have survived on bare rock.
Interspecific Competition Drives Change
As deeper soil develops, shrubs and small trees establish and outcompete earlier species for light, water and nutrients. Each seral stage makes conditions more suitable for the next species and often less suitable for itself.
Increasing Biodiversity
At each stage, the number of species increases, more niches become available (canopy, understory, leaf litter), and the food web becomes more complex. Species diversity peaks at or near the climax community.
Climax Community Reached
A stable, self-sustaining community that does not undergo further directional change. In lowland UK, the climatic climax is typically temperate deciduous woodland (e.g. oak woodland).
Secondary Succession
Secondary succession occurs where soil already exists – after fire, deforestation, abandonment of farmland, or flooding. Because soil is already present, secondary succession skips the pioneer stage on bare rock and begins at a later seral stage with grasses and herbaceous plants. It is significantly faster than primary succession.
Deflected Succession and Plagioclimax
When human activity (or other persistent factors) prevents a community from reaching its natural climatic climax, the resulting stable community is called a plagioclimax. This is maintained by deflected succession.
Examples include: moorland maintained by regular burning and grazing (would naturally become woodland), hay meadows maintained by annual mowing, and chalk grassland maintained by grazing sheep.
Eutrophication – The Exact Causal Chain for Full Marks
Eutrophication is one of the most frequently examined topics across all boards, and the mark scheme is brutally sequential. Each step must cause the next. Jumping from “fertilisers enter water” directly to “fish die” without the intermediate mechanism will typically earn 1 mark out of 5. Here is the complete sequence as it appears in mark schemes:
Fertiliser Leaching
Excess nitrates and/or phosphates from agricultural fertilisers are leached (washed) from soil into waterways by rain.
Algal Bloom
The increased concentration of mineral ions causes rapid growth of algae on the water surface (algal bloom). These are surface-dwelling, so they form a dense layer on top.
Light Blocked
The algal bloom blocks light from reaching submerged aquatic plants below the surface.
Submerged Plants Die
Without light, submerged plants cannot photosynthesise and die. This is a critical link – you must explain why plants die (no light → no photosynthesis), not just state it.
Decomposer Population Increases
Saprobionts (aerobic decomposing bacteria) break down the dead plant material. Their population increases rapidly due to the abundance of dead organic matter as a food source.
Dissolved Oxygen Depleted
The large population of saprobionts uses oxygen for aerobic respiration, increasing the biological oxygen demand (BOD). Dissolved oxygen concentration in the water falls dramatically.
Aerobic Organisms Die
Fish and other aerobic aquatic organisms die due to insufficient dissolved oxygen for aerobic respiration. The aquatic ecosystem collapses.

Struggling with Ecology? Let’s Fix That Together
Ecology questions demand precise causal chains and mark-scheme language. In a tutoring session, I will take you through exactly how I mark succession, eutrophication and energy transfer questions – showing you what earns each mark and what costs you marks.
Tyrone • CBiol MRSB • Former WJEC/Eduqas & Edexcel Examiner • 25+ Years Teaching
Book a Free 20-Minute ConsultationThe Nitrogen Cycle – Which Bacteria Your Board Requires
The nitrogen cycle is examined differently across boards, and this catches students who switch between specifications or use general revision resources. The sharpest divide is whether your board requires you to name specific bacteria. Getting this wrong wastes valuable revision time or, worse, leaves gaps in your knowledge.
The Four Key Processes
Nitrogen Fixation: N2 → NH3
Atmospheric nitrogen (N2) is converted to ammonia (NH3). This requires breaking the extremely stable triple bond in N2. Carried out by Rhizobium (symbiotic, in root nodules of legumes) and Azotobacter (free-living in soil). Also occurs via lightning and the Haber process industrially.
Nitrification: NH4+ → NO2− → NO3− (AEROBIC)
A two-step oxidation process. Nitrosomonas oxidises ammonium ions to nitrites. Nitrobacter oxidises nitrites to nitrates. Both are aerobic chemoautotrophs – they require oxygen. Plants absorb nitrate ions (NO3−) through their roots by active transport.
Ammonification: Organic N → NH4+
Saprobionts (decomposers) break down proteins and nucleic acids from dead organisms and animal waste, releasing ammonium ions (NH4+) into the soil. This feeds back into nitrification.
Denitrification: NO3− → N2 (ANAEROBIC)
Denitrifying bacteria (e.g. Pseudomonas) convert nitrates back into atmospheric nitrogen gas. This occurs in waterlogged, anaerobic conditions. This removes useful nitrogen from the soil – it is the reverse of nitrogen fixation and is undesirable for agriculture.
Which Board Requires Which Bacteria?
| Bacterium | Role | AQA | OCR A | Edexcel A | Edexcel B | WJEC/Eduqas |
|---|---|---|---|---|---|---|
| Nitrosomonas | NH4+ → NO2− | ✗ Not required | ✓ Required | ✗ Not required | ✗ Not required | ✓ Required |
| Nitrobacter | NO2− → NO3− | ✗ Not required | ✓ Required | ✗ Not required | ✗ Not required | ✓ Required |
| Rhizobium | N2 fixation (symbiotic) | ✗ Not required | ✓ Required | ✗ Not required | ✗ Not required | ✓ Required |
| Azotobacter | N2 fixation (free-living) | ✗ Not required | ✓ Required | ✗ Not required | ✗ Not required | ✓ Required |
| Pseudomonas | Denitrification | ✗ Not required | ✓ Required | ✗ Not required | ✗ Not required | ✓ Required |
The Phosphorus Cycle (AQA Only)
AQA is the only UK A-Level Biology board that explicitly requires the phosphorus cycle. Unlike nitrogen, the phosphorus cycle has no gaseous phase – phosphorus moves only through rock, soil, water and organisms.
Phosphate ions (PO43−) are released from rocks by weathering. Plants absorb phosphate ions from soil solution through their roots. Phosphorus passes through food chains in organic molecules (ATP, DNA, phospholipids). Decomposition returns phosphate to the soil. Mycorrhizae – mutualistic associations between fungi and plant roots – significantly increase phosphorus absorption by extending the effective root surface area.
Sampling Methods – When to Use Each Technique
The Eduqas 2024 examiner report specifically flagged that many candidates confused when to use transects versus randomly positioned quadrats. Choosing the wrong method, or failing to justify your choice, costs marks on practical methodology questions. Here is when to use each technique.
Quadrats (Random Sampling)
Used to estimate the abundance and distribution of sessile (non-moving) organisms across a habitat. Place a grid of numbered coordinates over the study area and use a random number generator to select positions for quadrat placement. Record frequency, density or percentage cover for each species. Calculate a mean from multiple quadrats.
Transects (Systematic Sampling)
Used to study changes in species distribution along an environmental gradient – for example, from the edge of a woodland to open grassland, or across a rocky shore from splash zone to low tide. A line transect records species touching the line at regular intervals. A belt transect uses continuous or interrupted quadrats along the line for more detailed data.
Mark-Release-Recapture (Motile Organisms)
Used to estimate the population size of mobile animals that cannot be counted directly. The method uses the Lincoln Index:
The Five Assumptions – And What Happens When They Fail
Examiners do not just want you to list assumptions. They want you to explain the consequences of each assumption being violated. This is where most students lose marks:
| Assumption | If Violated… | Effect on Estimate |
|---|---|---|
| No immigration or emigration between samples | Unmarked individuals enter → nm is too low | Overestimate |
| No births or deaths between samples | Deaths of marked individuals → nm is too low | Overestimate |
| Marking does not affect survival or behaviour | Marked organisms more easily predated → fewer recaptured | Overestimate |
| Marks do not wear off or fall off | Marked organisms appear unmarked → nm is too low | Overestimate |
| Sufficient time for random mixing | Marked individuals clumped → nm is too high or too low depending on recapture location | Inaccurate (either direction) |
Conservation vs Preservation – A Distinction Examiners Test Directly
Many students use “conservation” and “preservation” interchangeably. They are not the same thing, and examiners test the distinction directly, particularly on OCR A and Edexcel B papers.
Conservation
Active, sustainable management of ecosystems and resources. Conservation involves human intervention to maintain biodiversity while allowing sustainable use. Examples: coppicing woodland, controlled grazing, fishing quotas, seed banks, captive breeding programmes, SSSIs with management plans.
Preservation
Maintaining ecosystems in their present condition with minimal or no human interference. Preservation restricts human access and activity. Examples: restricting access to pristine wilderness areas, protecting ancient forest from any logging, marine no-take zones.
The key distinction is: conservation manages ecosystems (human involvement is planned and ongoing); preservation protects ecosystems (human involvement is minimised or excluded). OCR A mark schemes define conservation as maintaining “ecosystem/biodiversity/species/habitats” through “active/sustainable management” and preservation as leaving “ecosystems/habitats undisturbed.”
Exam Board Comparison – What YOUR Board Requires
This is the table I wish every student would check before starting ecology revision. The differences between boards are substantial – learning content your board does not examine wastes time, and missing content it does examine costs marks.
| Content Area | AQA | OCR A | OCR B | Edexcel A | Edexcel B | WJEC/Eduqas |
|---|---|---|---|---|---|---|
| Named N-cycle bacteria | ✗ | ✓ (5 genera) | ✓ (likely 3+) | ✗ | ✗ | ✓ (5 genera) |
| Phosphorus cycle | ✓ Required | ✗ | ✗ | ✗ | ✗ | ✗ |
| Mycorrhizae | ✓ Required | ✓ Required | ✗ | ✗ | ✗ | ✗ |
| Deflected succession | ✗ | ✓ Required | ✓ | ✗ | ✓ Required | ✗ |
| Simpson’s Index | ✗ | ✓ (in 4.2.1) | ✓ | ✓ (in Topic 4) | ✓ | ✓ |
| N = I − (F + R) | ✓ | ✓ | ✗ | ✗ | ✗ | ✓ |
| Conservation vs preservation | Implied | ✓ Explicit | ✓ | Partial | ✓ Explicit | ✓ Explicit |
| Mark-release-recapture | ✓ | ✓ | ✓ | Not in Topic 5 | ✓ | ✓ |
| Climate change focus | Limited | Limited | ✓ | ✓ Major | ✓ | ✓ |
| CITES / IUCN Red List | ✗ | ✗ | ✗ | ✗ | ✓ Required | ✗ |
10 Common Mistakes Examiners See Every Year
These are not hypothetical errors. These are mistakes I see repeatedly when marking ecology scripts, confirmed by examiner reports from AQA, OCR, WJEC/Eduqas and Edexcel. If you can eliminate these ten errors, your ecology marks will improve significantly.
| # | The Mistake | The Correction |
|---|---|---|
| 1 | Saying energy is “lost” between trophic levels | Energy is transferred to the surroundings as heat during respiration. It is not lost – it is transferred. |
| 2 | Saying energy is “produced” by photosynthesis | Light energy is converted to chemical energy in organic molecules. Energy cannot be produced or destroyed. |
| 3 | Confusing GPP and NPP in calculations | GPP = total energy fixed by photosynthesis. NPP = GPP − R (what remains after producer respiration). NPP is available to the next trophic level. |
| 4 | Vague succession answers: “bigger plants grow” | Explain the mechanism: pioneers form soil/humus, changed conditions allow new species, which outcompete previous species for light/nutrients. |
| 5 | Jumbled eutrophication – skipping steps | Each step must cause the next. The full chain is: leaching → algal bloom → light blocked → plants die → decomposers increase → oxygen depleted → fish die. |
| 6 | Stating denitrification needs oxygen | Denitrifying bacteria are anaerobic. Nitrifying bacteria (Nitrosomonas, Nitrobacter) are aerobic. Students swap these every year. |
| 7 | Confusing “community” with “population” | Population = one species. Community = all species in an area. This is directly tested in definition questions. |
| 8 | Listing mark-release-recapture assumptions without consequences | State the assumption AND explain what happens to the estimate if it is violated. Most violations lead to overestimation (nm too low). |
| 9 | Using transects when quadrats are appropriate | Transects study change along a gradient. Randomly placed quadrats estimate overall biodiversity. The Eduqas 2024 report flagged this specific confusion. |
| 10 | Weak percentage calculations | Always show working. Efficiency = (energy at level n+1 ÷ energy at level n) × 100. Eduqas 2024 noted a “surprising number” of students struggled with this. |
Frequently Asked Questions – A-Level Biology Ecology
Gross primary productivity (GPP) is the total rate of energy fixation by producers through photosynthesis. Net primary productivity (NPP) is what remains after subtracting the energy producers use for their own respiration: NPP = GPP − R. NPP represents the energy stored in plant biomass that is available to primary consumers. In calculation questions, read carefully whether you are given GPP or NPP – using the wrong one is the single most common calculation error in ecology.
Structure your answer as a causal chain, not a description. Name pioneer species (lichens, mosses). Explain HOW they change abiotic conditions (weather rock, form humus, add organic matter). State that changed conditions allow new species to colonise. Explain that new species outcompete earlier species for light and nutrients. State that species diversity increases at each stage as more niches become available. Conclude with the climax community as a stable, self-sustaining endpoint. Each step must logically cause the next.
This depends entirely on your board. OCR A and WJEC/Eduqas require all five genera: Nitrosomonas, Nitrobacter, Rhizobium, Azotobacter and Pseudomonas. AQA explicitly states that names of individual bacterial species are not required. Neither Edexcel A nor Edexcel B requires named bacteria. Always check your specification – learning unnecessary content wastes revision time.
The mark scheme expects seven linked steps: (1) excess nitrates and phosphates leach into waterways, (2) causing rapid algal growth on the surface, (3) which blocks light from reaching submerged plants, (4) submerged plants die because they cannot photosynthesise, (5) saprobionts (decomposing bacteria) multiply to break down dead material, (6) these saprobionts use oxygen for aerobic respiration causing dissolved oxygen levels to fall, (7) aerobic organisms such as fish die from lack of oxygen. Every step must cause the next – the connecting logic is what earns marks.
Energy is transferred to the surroundings as heat during respiration at every trophic level – this is the largest component. Additionally, not all organisms at one level are consumed by the next level (some die and are decomposed). Of the material that is consumed, not all is digested – some is egested in faeces. The energy in urine and faeces is not available to that trophic level. These combined losses mean only approximately 10% of energy at one level is incorporated into biomass at the next level.
Conservation is active, sustainable management of ecosystems – it involves planned human intervention such as coppicing, controlled grazing or fishing quotas. Preservation means maintaining ecosystems with minimal or no human interference, essentially leaving them untouched. This distinction is explicitly examined by OCR A, OCR B, Edexcel B, WJEC and Eduqas. AQA covers conservation methods but does not formally require the distinction with preservation.
The key is understanding that the population estimate N is calculated as (n₁ × n₂) ÷ nₓ. Most assumption violations reduce nₓ (the number of marked individuals recaptured), which makes N larger – an overestimate. For example, if marking increases predation risk, fewer marked organisms survive to be recaptured, so nₓ is smaller and the estimated population is larger than reality. Always state the specific assumption, explain the mechanism of violation, and state whether this causes an overestimate or underestimate.
Yes. Among UK A-Level Biology specifications, AQA is the only board that explicitly requires the phosphorus cycle as a standalone topic (in Topic 3.5.4 alongside the nitrogen cycle). No other board – OCR A, OCR B, Edexcel A, Edexcel B, WJEC or Eduqas – names the phosphorus cycle as a specification requirement. AQA also uniquely requires mycorrhizae in the context of phosphorus absorption, though OCR A requires mycorrhizae in their ecosystems module.

Need Help with A-Level Biology Ecology?
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Book a Free Consultation Email TyroneThese revision notes are intended to supplement your studies, not replace your textbook or teacher. Always refer to your specific exam board’s specification and past papers for the most accurate guidance on examined content. Biology Education is not affiliated with AQA, OCR, Edexcel, WJEC, Eduqas, Cambridge International or the IB.
